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Lookup NU author(s): Rachel Little,
Dr Colette Whitfield,
Dr Eimer Tuite,
Dr Andrew Pike
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© 2018, Springer Science+Business Media, LLC, part of Springer Nature. The synthesis of designer DNA requires an approach where the user can determine both the sequence and the number of nucleobases. The protocol outlined here describes an enzymatic method for the synthesis of long repeat-sequence DNA. The method utilizes a PCR-based approach; starting with short oligo-seeds, c.a. 20 bp, bearing a minimum of two repeating units of >8 bp sequences. During each heat-cool cycle, the oligo-seeds reanneal imperfectly leaving an overhang, which is then extended by the polymerase. The final length of the DNA is determined by the number of heat-cool cycles performed, reaching up to 20,000 bp after 20 cycles.
Author(s): Little RC, Whitfield CJ, Tuite EM, Pike AR
Publication type: Book Chapter
Publication status: Published
Book Title: DNA Nanotechnology
Online publication date: 21/06/2018
Acceptance date: 02/04/2018
Series Title: Methods in Molecular Biology
Publisher: Humana Press Inc.
Place Published: New York, NY, USA
Library holdings: Search Newcastle University Library for this item