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Lookup NU author(s): Professor Graham BonwickORCiD
This is the authors' accepted manuscript of a conference proceedings (inc. abstract) that has been published in its final definitive form by UCT Prague Press, 2015.
For re-use rights please refer to the publisher's terms and conditions.
Aptamers are short oligonucleotides comprised of fixed primer binding regions and random sequence regions. Through a process known as Systematic Evolution of Ligands by Exponential Enrichment (SELEX), aptamers may be selected for a variety of target molecules such as proteins, peptides, toxins, viruses and whole cells. This effectively facilitates their use as biomolecular ligands similar to antibodies. Aptamers can offer several advantages over antibodies such as in vitro production thus avoiding ethical implications as with antibodies, ease of labelling with little or no alteration to binding sites, reduced production cost and longer shelf life through greater thermotolerance. Data are presented that illustrate performance optimisation of conventional SELEX procedures for L. monocytogenes. Novel methods that include either direct modification of the SELEX process or the smart use of ligands in molecular diagnostic methods are described. These methods have demonstrated an ability to improve the performance (target affinity and specificity) of oligonucleotide aptamers and contribute to improved sensitivity of derived assays. The effect of culture media on the recognition of L. monocytogenes by the aptamers is also reported.
Author(s): Gornall A, Coleman R, Birch C, Bonwick GA
Editor(s): Pulkrabová, J; Tomaniová, M; Nielen, M; Hajslova, J
Publication type: Conference Proceedings (inc. Abstract)
Publication status: Published
Conference Name: 7th International Symposium on Recent Advances in Food Analysis
Year of Conference: 2015
Print publication date: 03/11/2015
Acceptance date: 04/09/2015
Date deposited: 14/06/2019
Publisher: UCT Prague Press
