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Expression cloning human and rat renal cortex Na/Pi cotransporters: behind the scenes in the Murer laboratory

Lookup NU author(s): Dr Andreas Werner

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This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).


Abstract

In the pre-genomic era, the cloning of a cDNA represented a significant achievement, particularly if the gene of interest encoded a membrane protein. At the time, molecular probes such as partial peptide sequences, suitable nucleic acid sequences, or antibodies were unavailable for most proteins and the “sodium-phosphate transporter” was no exception. In contrast, brush-border membrane vesicles and epithelial cell culture experiments had established a reliable set of functional hallmarks that described Na-dependent phosphate transport activity in some detail. Moreover, aspects of hormonal regulation of phosphate homeostasis could be recapitulated in these model systems. Expression cloning elegantly combined functional protein expression in Xenopus laevis oocytes with molecular biology to overcome the lack of molecular probes.


Publication metadata

Author(s): Magagnin S, Werner A

Publication type: Review

Publication status: Published

Journal: Pflügers Archiv - European Journal of Physiology

Year: 2018

Online publication date: 03/09/2018

Acceptance date: 15/08/2018

ISSN (print): 0031-6768

ISSN (electronic): 1432-2013

URL: https://doi.org/10.1007/s00424-018-2198-9

DOI: 10.1007/s00424-018-2198-9


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