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Lookup NU author(s): Dr Andreas Werner
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
In the pre-genomic era, the cloning of a cDNA represented a significant achievement, particularly if the gene of interest encoded a membrane protein. At the time, molecular probes such as partial peptide sequences, suitable nucleic acid sequences, or antibodies were unavailable for most proteins and the “sodium-phosphate transporter” was no exception. In contrast, brush-border membrane vesicles and epithelial cell culture experiments had established a reliable set of functional hallmarks that described Na-dependent phosphate transport activity in some detail. Moreover, aspects of hormonal regulation of phosphate homeostasis could be recapitulated in these model systems. Expression cloning elegantly combined functional protein expression in Xenopus laevis oocytes with molecular biology to overcome the lack of molecular probes.
Author(s): Magagnin S, Werner A
Publication type: Review
Publication status: Published
Journal: Pflügers Archiv - European Journal of Physiology
Online publication date: 03/09/2018
Acceptance date: 15/08/2018
ISSN (print): 0031-6768
ISSN (electronic): 1432-2013