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Constriction Rate Modulation Can Drive Cell Size Control and Homeostasis in C. crescentus

Lookup NU author(s): Dr Seamus Holden

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Abstract

Rod-shaped bacteria typically grow first via sporadic and dispersed elongation along their lateral walls and then via a combination of zonal elongation and constriction at the division site to form the poles of daughter cells. Although constriction comprises up to half of the cell cycle, its impact on cell size control and homeostasis has rarely been considered. To reveal the roles of cell elongation and constriction in bacterial size regulation during cell division, we captured the shape dynamics of Caulobacter crescentus with time-lapse structured illumination microscopy and used molecular markers as cell-cycle landmarks. We perturbed the constriction rate using a hyperconstriction mutant or fosfomycin ([(2R,3S)-3-methyloxiran-2-yl]phosphonic acid) inhibition. We report that the constriction rate contributes to both size control and homeostasis, by determining elongation during constriction and by compensating for variation in pre-constriction elongation on a single-cell basis.

Publication metadata

Author(s): Lambert A, Vanhecke A, Archetti A, Holden S, Schaber F, Pincus Z, Laub MT, Goley E, Manley S

Publication type: Article

Publication status: Published

Journal: iScience

Year: 2018

Volume: 4

Pages: 180-189

Print publication date: 29/06/2018

Online publication date: 30/05/2018

Acceptance date: 24/05/2018

Date deposited: 18/09/2018

ISSN (electronic): 2589-0042

Publisher: Cell Press

URL: https://doi.org/10.1016/j.isci.2018.05.020

DOI: 10.1016/j.isci.2018.05.020

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