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Simultaneous Electrochemical Detection of Glucose and Non-Esterified Fatty Acids (NEFAs) for Diabetes Management

Lookup NU author(s): Samet Sahin, James Merotra, Dr Jing Kang, Professor Michael Trenell, Professor Mike Catt, Dr Eileen Yu

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Abstract

IEEE In this work, the feasibility of simultaneous electrochemical detection of glucose and non-esterified fatty acids (NEFA) is investigated using enzyme-based biosensors. The detection of glucose is based on ferrocene-Nafion-glucose oxidase (Fc-Nafion-GOx) modified screen-printed electrodes (SPEs). Solutions of Fc-Nafion-GOx were drop casted on SPEs and dried at room temperature allowing immobilisation of GOx entrapped in Nafion polymer with Fc. The detection of NEFA is based on bienzyme electrodes fabricated by the layer by layer (LbL) technique applied onto SPEs using multilayers of poly(dimethyldiallyammonium chloride) polymer, multi-walled carbon nanotube composite films and two enzymes: acyl-CoA synthetase and acyl-CoA oxidase. Oleoyl coenzyme A lithium salt (OACoA) and oleic acid (OA) were used as NEFA of interests because they are two of the most employed markers in clinical tests. Preliminary results demonstrated that enzyme electrodes showed linear response within the physiological concentration range of glucose and OA in human blood plasma as 5 mM and 1.2 mM, respectively. These results indicate a promising multibiosensor platform for rapid one-step determination of glucose and NEFA simultaneously. This could lead the way towards the fabrication of glucose-NEFA biosensors which can be important in diabetes management and early diagnosis.


Publication metadata

Author(s): Sahin S, Merotra J, Kang J, Trenell M, Catt M, Yu EH

Publication type: Article

Publication status: Published

Journal: IEEE Sensors Journal

Year: 2018

Volume: 18

Issue: 22

Pages: 9075-9080

Print publication date: 15/11/2018

Online publication date: 17/09/2018

Acceptance date: 02/04/2018

ISSN (print): 1530-437X

ISSN (electronic): 1558-1748

Publisher: IEEE

URL: https://doi.org/10.1109/JSEN.2018.2870071

DOI: 10.1109/JSEN.2018.2870071


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