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Lookup NU author(s): Dr Colette Whitfield,
Professor Bernard Connolly,
Dr Eimer Tuite,
Dr Andrew Pike
This is the authors' accepted manuscript of an article that has been published in its final definitive form by Wiley-VCH Verlag, 2018.
For re-use rights please refer to the publisher's terms and conditions.
© 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim The self-priming synthesis of multiply modified DNA by the extension of repeating unit duplex “oligoseeds” provides a source of versatile DNA. Sterically-demanding nucleotides 5-Br-dUTP, 7-deaza-7-I-dATP, 6-S-dGTP, 5-I-dCTP as well as 5-(octadiynyl)-dCTP were incorporated into two extending oligoseeds; [GATC]5/[GATC]5 and [A4G]4/[CT4]4. The products contained modifications on one or both strands of DNA, demonstrating their recognition by the polymerase as both template (reading) and substrate (writing). Nucleobase modifications that lie in the major groove were reliably read and written by the polymerase during the extension reaction, even when bulky or in contiguous sequences. Repeat sequence DNA over 500 bp long, bearing four different modified units was produced by this method. The number, position and type of modification, as well as the overall length of the DNA can be controlled to yield designer DNA that offers sequence-determined sites for further chemical adaptations, targeted small molecule binding studies, or sensing and sequencing applications.
Author(s): Whitfield CJ, Little RC, Khan K, Ijiro K, Connolly BA, Tuite EM, Pike AR
Publication type: Article
Publication status: Published
Journal: Chemistry - A European Journal
Print publication date: 12/10/2018
Online publication date: 21/06/2018
Acceptance date: 19/06/2018
Date deposited: 01/01/2019
ISSN (print): 0947-6539
ISSN (electronic): 1521-3765
Publisher: Wiley-VCH Verlag
Notes: This project has received funding from the European Union's Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No 778001.
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