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Lookup NU author(s): Dr Emanuela Torelli
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A prototype for a DNA origami nanorobot has been designed, produced and tested. The 3D cylindrical nanorobot (14x14x48 nm) with a switchable flap, is able to detect picomol amounts of a DNA target and reveal it using a DNAzyme as the biocatalytic label.The actuation switch is triggered by an external nucleic acid (red) that interacts with a complementary nucleic acid (yellow) that is beard externally by the nanostructure.[1,2]Upon actuation the nanorobot moves the flap and a DNAzyme (green) was pulled out of the cylinder and exposed to hemin (gold): the hemin/G-quadruplex horseradish peroxidase mimicking DNAzyme catalyzes a colorimetric reaction or chemiluminescence generation.[1]The nanorobotinternal cavity (3 zeptoliters) is large enough to accomodate a functional cargo, while the nano-object is small enough to be entirely contained within the capsid of viruses.[3]This work opens perspectives for the construction of nanorobots that can be hosted within cellular delivery vectors and can host, concurrently, selectively accessible molecular payloads in their internal cavity.References[1] E. Torelli et al. 2014. A DNA Origami Nanorobot Controlled by Nucleic Acid Hybridization. Small. First published on line. [2] M. Marini et al. 2011. A Revertible, Autonomous, Self-assembled DNA-Origami Nanoactuator. Nano Lett. 11, 5449-5454. [3] J. Mikkila et al. 2014. Virus encapsulated DNA origami nanostructures for cellular delivery. Nano Lett. 14: 2196-2200.
Author(s): Torelli E, Marini M, Palmano S, Piantanida L, Polano C, Scarpellini A, Lazzarino M, Firrao G
Editor(s): Ricci, F.
Publication type: Conference Proceedings (inc. Abstract)
Publication status: Published
Conference Name: Functional DNA Nanotechnology
Year of Conference: 2014
Acceptance date: 14/04/2014
