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NIPAM-based Microgel Microenvironment Regulates the Therapeutic Function of Cardiac Stromal Cells

Lookup NU author(s): Professor Sheng Dai

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Abstract

© 2018 American Chemical Society. To tune the chemical, physical, and mechanical microenvironment for cardiac stromal cells to treat acute myocardial infarction (MI), we prepared a series of thermally responsive microgels with different surface charges (positive, negative, and neutral) and different degrees of hydrophilicity, as well as functional groups (carboxyl, hydroxyl, amino, and methyl). These microgels were used as injectable hydrogels to create an optimized microenvironment for cardiac stromal cells (CSCs). Our results indicated that a hydrophilic and negatively charged microenvironment created from poly(N-isopropylacrylamide-co-itaconic acid) was favorable for maintaining high viability of CSCs, promoting CSC proliferation and facilitating the formation of CSC spheroids. A large number of growth factors, such as vascular endothelial growth factor (VEGF), insulin-like growth factor I (IGF-1), and stromal-derived factor-1 (SDF-1) were released from the spheroids, promoting neonatal rat cardiomyocyte activation and survival. After injecting the poly(N-isopropylacrylamide-co-itaconic acid) microgel into mice, we examined their acute inflammation and T-cell immune reactions. The microgel itself did not elicit obvious immune response. We then injected the same microgel-encapsulated with CSCs into MI mice. The result revealed the treatment-promoted MI heart repair through angiogenesis and inhibition of apoptosis with an improved cell retention rate. This study will open a door for tailoring poly(N-isopropylacrylamide)-based microgel as a delivery vehicle for CSC therapy.

Publication metadata

Author(s): Cui X, Tang J, Hartanto Y, Zhang J, Bi J, Dai S, Qiao SZ, Cheng K, Zhang H

Publication type: Article

Publication status: Published

Journal: ACS Applied Materials and Interfaces

Year: 2018

Volume: 10

Issue: 44

Pages: 37783-37796

Print publication date: 07/11/2018

Online publication date: 12/10/2018

Acceptance date: 12/10/2018

ISSN (print): 1944-8244

ISSN (electronic): 1944-8252

Publisher: American Chemical Society

URL: https://doi.org/10.1021/acsami.8b09757

DOI: 10.1021/acsami.8b09757

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