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Lookup NU author(s): Heather Crawford, Professor Majlinda LakoORCiD, Dr Nicholas Hole, Professor Colin Jahoda
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
© 2018 Jun Liu et al. In the hair follicle, the dermal papilla (DP) and dermal sheath (DS) support and maintain proliferation and differentiation of the epithelial stem cells that produce the hair fibre. In view of their regulatory properties, in this study, we investigated the interaction between hair follicle dermal cells (DP and DS) and embryonic stem cells (ESCs); induced pluripotent stem cells (iPSCs); and haematopoietic stem cells. We found that coculture of follicular dermal cells with ESCs or iPSCs supported their prolonged maintenance in an apparently undifferentiated state as established by differentiation assays, immunocytochemistry, and RT-PCR for markers of undifferentiated ESCs. We further showed that cytokines that are involved in ESC support are also expressed by cultured follicle dermal cells, providing a possible explanation for maintenance of ES cell stemness in cocultures. The same cytokines were expressed within follicles in situ in a pattern more consistent with a role in follicle growth activities than stem cell maintenance. Finally, we show that cultured mouse follicle dermal cells provide good stromal support for haematopoiesis in an established coculture model. Human follicular dermal cells represent an accessible and readily propagated source of feeder cells for pluripotent and haematopoietic cells and have potential for use in clinical applications.
Author(s): Liu J, Higgins CA, Whitehouse JC, Harris SJ, Crawford H, Christiano AM, Lako M, Hole N, Jahoda CAB
Publication type: Article
Publication status: Published
Journal: Stem Cells International
Year: 2018
Volume: 2018
Online publication date: 02/08/2018
Acceptance date: 26/04/2018
Date deposited: 10/01/2019
ISSN (print): 1687-966X
ISSN (electronic): 1687-9678
Publisher: Hindawi Limited
URL: https://doi.org/10.1155/2018/8631432
DOI: 10.1155/2018/8631432
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