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Mechanistic aspects of maltotriose-conjugate translocation to the Gram-negative bacteria cytoplasm

Lookup NU author(s): Dr Satya Bhamidimarri

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Abstract

© 2018 Dumont et al. Small molecule accumulation in Gram-negative bacteria is a key challenge to discover novel antibiotics, because of their two membranes and efflux pumps expelling toxic molecules. An approach to overcome this challenge is to hijack uptake pathways so that bacterial transporters shuttle the antibiotic to the cytoplasm. Here, we have characterized maltodextrin–fluorophore conjugates that can pass through both the outer and inner membranes mediated by components of the Escherichia coli maltose regulon. Single-channel electrophysiology recording demonstrated that the compounds permeate across the LamB channel leading to accumulation in the periplasm. We have also demonstrated that a maltotriose conjugate distributes into both the periplasm and cytoplasm. In the cytoplasm, the molecule activates the maltose regulon and triggers the expression of maltose binding protein in the periplasmic space indicating that the complete maltose entry pathway is induced. This maltotriose conjugate can (i) reach the periplasmic and cytoplasmic compartments to significant internal concentrations and (ii) auto-induce its own entry pathway via the activation of the maltose regulon, representing an interesting prototype to deliver molecules to the cytoplasm of Gram-negative bacteria.


Publication metadata

Author(s): Dumont E, Vergalli J, Pajovic J, Bhamidimarri SP, Morante K, Wang J, Lubriks D, Suna E, Stavenger RA, Winterhalter M, Refregiers M, Pages J-M

Publication type: Article

Publication status: Published

Journal: Life Science Alliance

Year: 2019

Volume: 2

Issue: 1

Print publication date: 01/02/2019

Online publication date: 28/12/2018

Acceptance date: 19/12/2018

ISSN (electronic): 2575-1077

Publisher: Life Science Alliance LLC

URL: https://doi.org/10.26508/lsa.201800242

DOI: 10.26508/lsa.201800242


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