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Cloning, expression, and purification of intact polyketide synthase modules

Lookup NU author(s): Dr James Stach, Dr Paul Race

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Abstract

© 2019 Elsevier Inc. Polyketides are a structurally and functionally diverse family of bioactive natural products that have proven to be a rich source of pharmaceutical and agrochemical lead compounds. Many polyketides are biosynthesized by large multifunctional megaenzymes termed type I modular polyketide synthases (PKSs). These systems possess a distinctive assembly line-like architecture, comprising a series of linearly arranged, multidomain extension modules, housed in sequence within giant polypeptide chains. Due to their inherently modular structures, PKSs represent attractive targets for reengineering, enabling access to functionally optimized “nonnatural” natural products. In this chapter we describe methods for the molecular cloning, recombinant over-expression, and purification of intact PKS modules and multimodular PKS polypeptides. The usefulness of these methods is demonstrated by applying them to the study of the abyssomicin C PKS, a > 1 MDa multimodular synthase responsible for the biosynthesis of a polyketide antimicrobial lead compound.


Publication metadata

Author(s): Maschio L, Parnell AE, Lees NR, Willis CL, Schaffitzel C, Stach JEM, Race PR

Publication type: Book Chapter

Publication status: Published

Book Title: Methods in Enzymology

Year: 2019

Volume: 617

Pages: 63-82

Online publication date: 04/02/2019

Acceptance date: 02/04/2018

Publisher: Academic Press

URL: https://doi.org/10.1016/bs.mie.2018.12.018

DOI: 10.1016/bs.mie.2018.12.018


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