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Organophosphate hydrolase is a lipoprotein and interacts with pi-specific transport system to facilitate growth of brevundimonas diminuta using op insecticide as source of phosphate

Lookup NU author(s): Professor Tracy Palmer FRS FRSE FMedSciORCiD

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This is the final published version of an article that has been published in its final definitive form by American Society for Biochemistry and Molecular Biology Inc., 2016.

For re-use rights please refer to the publisher's terms and conditions.


Abstract

©2016 by The American Society for Biochemistry and Molecular Biology, Inc. Organophosphate hydrolase (OPH), encoded by the organophosphate degradation (opd) island, hydrolyzes the triester bond found in a variety of organophosphate insecticides and nerve agents. OPH is targeted to the inner membrane of Brevundimonas diminuta in a pre-folded conformation by the twin arginine transport (Tat) pathway. The OPH signal peptide contains an invariant cysteine residue at the junction of the signal peptidase (Spase) cleavage site along with a well conserved lipobox motif. Treatment of cells producing native OPH with the signal peptidase II inhibitor globomycin resulted in accumulation of most of the pre-OPH in the cytoplasm with negligible processed OPH detected in the membrane. Substitution of the conserved lipobox cysteine to serine resulted in release of OPH into the periplasm, confirming that OPH is a lipoprotein. Analysis of purified OPH revealed that it was modified with the fatty acids palmitate and stearate. Membrane-bound OPH was shown to interact with the outer membrane efflux protein TolC and with PstS, the periplasmic component of the ABC transporter complex (PstSACB) involved in phosphate transport. Interaction ofOPHwith PstS appears to facilitate transport of Pi generated from organophosphates due to the combined action of OPH and periplasmically located phosphatases. Consistent with this model, opd null mutants of B. diminuta failed to grow using the organophosphate insecticide methyl parathion as sole source of phosphate.


Publication metadata

Author(s): Parthasarathy S, Parapatla H, Nandavaram A, Palmer T, Siddavattam D

Publication type: Article

Publication status: Published

Journal: Journal of Biological Chemistry

Year: 2016

Volume: 291

Issue: 14

Pages: 7774-7785

Print publication date: 01/04/2016

Online publication date: 09/02/2016

Acceptance date: 07/02/2016

Date deposited: 15/02/2019

ISSN (print): 0021-9258

ISSN (electronic): 1083-351X

Publisher: American Society for Biochemistry and Molecular Biology Inc.

URL: https://doi.org/10.1074/jbc.M116.715110

DOI: 10.1074/jbc.M116.715110

PubMed id: 26861877


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