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PCV2 replication promoted by oxidative stress is dependent on the regulation of autophagy on apoptosis

Lookup NU author(s): Dr Viktor Korolchuk, Dr Bernadette Carroll

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This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).

Abstract

© 2019 The Author(s).Porcine circovirus type 2 (PCV2) is an economically important swine pathogen but some extra trigger factors are required for the development of PCV2-associated diseases. By evaluating cap protein expression, viral DNA copies and the number of infected cells, the present study further confirmed that oxidative stress can promote PCV2 replication. The results showed that oxidative stress induced autophagy in PCV2-infected PK15 cells. Blocking autophagy with inhibitor 3-methyladenine or ATG5-specific siRNA significantly inhibited oxidative stress-promoted PCV2 replication. Importantly, autophagy inhibition significantly increased apoptosis in oxidative stress-treated PK15 cells. Suppression of apoptosis by benzyloxycarbonyl-Val-Ala-Asp fluoromethylketone in conditions of autophagy inhibition restored PCV2 replication. Taken together, autophagy protected host cells against potential apoptosis and then contributed to PCV2 replication promotion caused by oxidative stress. Our findings can partly explain the pathogenic mechanism of PCV2 related to the oxidative stress-induced autophagy.

Publication metadata

Author(s): Zhai N, Liu K, Li H, Liu Z, Wang H, Korolchuk VI, Carroll B, Pan C, Gan F, Huang K, Chen X

Publication type: Article

Publication status: Published

Journal: Veterinary Research

Year: 2019

Volume: 50

Online publication date: 05/03/2019

Acceptance date: 14/02/2019

Date deposited: 20/03/2019

ISSN (print): 0928-4249

ISSN (electronic): 1297-9716

Publisher: BioMed Central Ltd

URL: https://doi.org/10.1186/s13567-019-0637-z

DOI: 10.1186/s13567-019-0637-z

PubMed id: 30836990

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