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Biomimetic Bacterial Identification Platform Based on Thermal Wave Transport Analysis (TWTA) through Surface-Imprinted Polymers

Lookup NU author(s): Dr Marloes Peeters

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND).


Abstract

© 2017 American Chemical Society. This paper introduces a novel bacterial identification assay based on thermal wave analysis through surface-imprinted polymers (SIPs). Aluminum chips are coated with SIPs, serving as synthetic cell receptors that have been combined previously with the heat-transfer method (HTM) for the selective detection of bacteria. In this work, the concept of bacterial identification is extended toward the detection of nine different bacterial species. In addition, a novel sensing approach, thermal wave transport analysis (TWTA), is introduced, which analyzes the propagation of a thermal wave through a functional interface. The results presented here demonstrate that bacterial rebinding to the SIP layer resulted in a measurable phase shift in the propagated wave, which is most pronounced at a frequency of 0.03 Hz. In this way, the sensor is able to selectively distinguish between the different bacterial species used in this study. Furthermore, a dose-response curve was constructed to determine a limit of detection of 1 × 104 CFU mL-1, indicating that TWTA is advantageous over HTM in terms of sensitivity and response time. Additionally, the limit of selectivity of the sensor was tested in a mixed bacterial solution, containing the target species in the presence of a 99-fold excess of competitor species. Finally, a first application for the sensor in terms of infection diagnosis is presented, revealing that the platform is able to detect bacteria in clinically relevant concentrations as low as 3 × 104 CFU mL-1 in spiked urine samples.


Publication metadata

Author(s): Steen Redeker E, Eersels K, Akkermans O, Royakkers J, Dyson S, Nurekeyeva K, Ferrando B, Cornelis P, Peeters M, Wagner P, Dilien H, Van Grinsven B, Cleij TJ

Publication type: Article

Publication status: Published

Journal: ACS Infectious Diseases

Year: 2017

Volume: 3

Issue: 5

Pages: 388-397

Print publication date: 12/05/2017

Online publication date: 07/04/2017

Acceptance date: 22/03/2017

Date deposited: 15/04/2019

ISSN (electronic): 2373-8227

Publisher: American Chemical Society

URL: https://doi.org/10.1021/acsinfecdis.7b00037

DOI: 10.1021/acsinfecdis.7b00037

PubMed id: 28388095


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