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Heat transfer resistance as a tool to quantify hybridization efficiency of DNA on a nanocrystalline diamond surface

Lookup NU author(s): Dr Marloes Peeters

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Abstract

In this article, we report on a label-free real-time method based on heat transfer resistivity for thermal monitoring of DNA denaturation and its potential to quantify DNA fragments with a specific sequence of interest. Probe DNA, consisting of a 36-mer fragment was covalently immobilized on a nanocrystalline diamond surface, created by chemical vapor deposition on a silicon substrate. Various concentrations of full matched 29-mer target DNA fragments were hybridized with this probe DNA. We observed that the change in heat transfer resistance upon denaturation depends on the concentration of target DNA used during the hybridization, which allowed us to determine the dose-response curve. Therefore, these results illustrate the potential of this technique to quantify the concentration of a specific DNA fragment and to quantify the hybridization efficiency to its probe. © 2014 Elsevier B.V.


Publication metadata

Author(s): Cornelis P, Vandenryt T, Wackers G, Kellens E, Losada-Perez P, Thoelen R, De Ceuninck W, Eersels K, Drijkoningen S, Haenen K, Peeters M, Van Grinsven B, Wagner P

Publication type: Article

Publication status: Published

Journal: Diamond and Related Materials

Year: 2014

Volume: 48

Pages: 32-36

Print publication date: 01/09/2014

Online publication date: 28/06/2014

Acceptance date: 23/06/2014

ISSN (print): 0925-9635

Publisher: Elsevier Ltd

URL: https://doi.org/10.1016/j.diamond.2014.06.008

DOI: 10.1016/j.diamond.2014.06.008


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