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Heat-transfer-based detection of l-nicotine, histamine, and serotonin using molecularly imprinted polymers as biomimetic receptors

Lookup NU author(s): Dr Marloes Peeters

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Abstract

In this work, we will present a novel approach for the detection of small molecules with molecularly imprinted polymer (MIP)-type receptors. This heat-transfer method (HTM) is based on the change in heat-transfer resistance imposed upon binding of target molecules to the MIP nanocavities. Simultaneously with that technique, the impedance is measured to validate the results. For proof-of-principle purposes, aluminum electrodes are functionalized with MIP particles, and l-nicotine measurements are performed in phosphate-buffered saline solutions. To determine if this could be extended to other templates, histamine and serotonin samples in buffer solutions are also studied. The developed sensor platform is proven to be specific for a variety of target molecules, which is in agreement with impedance spectroscopy reference tests. In addition, detection limits in the nanomolar range could be achieved, which is well within the physiologically relevant concentration regime. These limits are comparable to impedance spectroscopy, which is considered one of the state-of-the-art techniques for the analysis of small molecules with MIPs. As a first demonstration of the applicability in biological samples, measurements are performed on saliva samples spiked with l-nicotine. In summary, the combination of MIPs with HTM as a novel readout technique enables fast and low-cost measurements in buffer solutions with the possibility of extending to biological samples. [Figure not available: see fulltext.] © 2013 Springer-Verlag Berlin Heidelberg.


Publication metadata

Author(s): Peeters M, Csipai P, Geerets B, Weustenraed A, Van Grinsven B, Thoelen R, Gruber J, De Ceuninck W, Cleij TJ, Troost FJ, Wagner P

Publication type: Article

Publication status: Published

Journal: Analytical and Bioanalytical Chemistry

Year: 2013

Volume: 405

Issue: 20

Pages: 6453-6460

Print publication date: 01/08/2013

Online publication date: 18/05/2013

ISSN (print): 1618-2642

ISSN (electronic): 1618-2650

Publisher: Springer

URL: https://doi.org/10.1007/s00216-013-7024-9

DOI: 10.1007/s00216-013-7024-9

PubMed id: 23685906


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