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Molecular microbial ecology of stable versus failing rice straw anaerobic digesters

Lookup NU author(s): Dr Andrew Zealand, Professor Tony Roskilly, Professor David Graham

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This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).


Abstract

Waste rice straw (RS) is generated in massive quantities around the world and is often burned, creating greenhouse gas and air quality problems. Anaerobic digestion (AD) may be a better option for RS management, but RS is presumed to be comparatively refractory under anaerobic conditions without pre‐treatment or co‐substrates. However, this presumption assumes frequent reactor feeding regimes but less frequent feeding may be better for RS due to slow hydrolysis rates. Here, we assess how feeding frequency (FF) and organic loading rate (OLR) impacts microbial communities and biogas production in RS AD reactors. Using 16S rDNA amplicon sequencing and bioinformatics, microbial communities from five bench‐scale bioreactors were characterized. At low OLR (1.0 g VS l−1 day−1), infrequently fed units (once every 21 days) had higher specific biogas yields than more frequent feeding (five in 7 days), although microbial community diversities were statistically similar (P > 0.05; ANOVA with Tukey comparison). In contrast, an increase in OLR to 2.0 g VS l−1 day−1 significantly changed Archaeal and fermenting Eubacterial sub‐communities and the least frequency fed reactors failed. ‘Stable’ reactors were dominated by Methanobacterium, Methanosarcina and diverse Bacteroidetes, whereas ‘failed’ reactors saw shifts towards Clostridia and Christensenellaceae among fermenters and reduced methanogen abundances. Overall, OLR impacted RS AD microbial communities more than FF. However, combining infrequent feeding and lower OLRs may be better for RS AD because of higher specific yields.


Publication metadata

Author(s): Zealand AM, Mei R, Roskilly AP, Liu WT, Graham DW

Publication type: Article

Publication status: Published

Journal: Microbial Biotechnology

Year: 2019

Volume: 12

Issue: 5

Pages: 879-891

Print publication date: 01/09/2019

Online publication date: 10/06/2019

Acceptance date: 14/05/2019

Date deposited: 12/06/2019

ISSN (electronic): 1751-7915

Publisher: Wiley-Blackwell Publishing Ltd.

URL: https://doi.org/10.1111/1751-7915.13438

DOI: 10.1111/1751-7915.13438


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