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Differential proteomic analysis of midguts from Nosema ceranae-infected honeybees reveals manipulation of key host functions

Lookup NU author(s): Dr Johan Panek

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Abstract

Many invasive pathogens effectively bypass the insect defenses to ensure the completion of their life cycle. Among those, an invasive microsporidian species, Nosema ceranae, can cause nosemosis in honeybees. N. ceranae was first described in the Asian honeybee Apis cerana and is suspected to be involved in Western honeybee (Apis mellifera) declines worldwide. The midgut of honeybees is the first barrier against N. ceranae attacks. To bring proteomics data on honeybee/N. ceranae crosstalk and more precisely to decipher the worker honeybee midgut response after an oral inoculation of N. ceranae (10 days post-infection), we used 2D-DIGE (2-Dimensional Differential In-Gel Electrophoresis) combined with mass spectrometry. Forty-five protein spots produced by the infected worker honeybee group were shown to be differentially expressed when compared to the uninfected group; 14 were subsequently identified by mass spectrometry. N. ceranae mainly caused a modulation of proteins involved in three key host biological functions: (i) energy production, (ii) innate immunity (reactive oxygen stress) and (iii) protein regulation. The modulation of these host biological functions suggests that N. ceranae creates a zone of “metabolic habitat modification” in the honeybee midgut favoring its development by enhancing availability of nutrients and reducing the worker honeybee defense.


Publication metadata

Author(s): Vidau C, Panek J, Texier C, Biron DG, Belzunces LP, Le Gall M, Broussard C, Delbac F, El Alaoui H

Publication type: Article

Publication status: Published

Journal: Journal of Invertebrate Pathology

Year: 2014

Volume: 121

Pages: 89-96

Print publication date: 01/09/2014

Online publication date: 16/07/2014

Acceptance date: 08/07/2014

ISSN (print): 0022-2011

ISSN (electronic): 1096-0805

Publisher: Academic Press

URL: https://doi.org/10.1016/j.jip.2014.07.002

DOI: 10.1016/j.jip.2014.07.002


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