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Lookup NU author(s): Dr John TaylorORCiD, Dr Susan BissettORCiD, Dr Deepan Shah, Professor Philip Preshaw
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
Periodontitis is an economically important disease which is highly prevalent worldwide. current diagnostic approaches are time-consuming and require interpretation of multiple aspects of clinical and radiographic assessment. Chair-side monitoring of inflammatory mediators of periodontitis could provide immediate information about disease activity, which can inform patient management. We aimed to develop a novel prototype biosensor to measure salivary matrix metalloproteinase-8 (MMP-8) using specific antibodies and surface acoustic wave (SAW) technology. The analytical performance of the prototype biosensor was compared to standard enzyme-linked immunosorbent assay (ELISA) using unstimulated saliva samples obtained from patients with periodontitis before and after non-surgical treatment (N = 58), patients with gingivitis (N = 54) and periodontally healthy volunteers (N = 65). Receiver operator characteristic (ROC) analysis for distinguishing periodontitis from health revealed an almost identical performance between the sensor and ELISA assays (area under curve values (AUC): ELISA 0.93; SAW 0.89). Furthermore, both analytical approaches yielded readouts which distinguished between heath, gingivitis and periodontitis, correlated identically with clinical measures of periodontal disease and recorded similar post-treatment decreases in salivary MMP-8 in periodontitis. The assay time for our prototype device is 20 minutes. The prototype SAW biosensor is a novel and rapid method of monitoring periodontitis which delivers similar analytical performance to conventional laboratory assays.
Author(s): Taylor JJ, Jaedicke KJ, vandeMerwe RC, Bissett SM, Landsdowne N, Whall KM, Pickering K, Thornton V, Lawson V, Yatsuda H, Kogai T, Shah D, Athey D, Preshaw PM
Publication type: Article
Publication status: Published
Journal: Scientific Reports
Year: 2019
Volume: 9
Print publication date: 30/07/2019
Online publication date: 30/07/2019
Acceptance date: 11/07/2019
Date deposited: 31/07/2019
ISSN (electronic): 2045-2322
Publisher: Springer Nature Publishing
URL: https://doi.org/10.1038/s41598-019-47513-w
DOI: 10.1038/s41598-019-47513-w
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