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Cross-Interaction Chromatography-Based QSAR Model for Early-Stage Screening to Facilitate Enhanced Developability of Monoclonal Antibody Therapeutics

Lookup NU author(s): Arathi Kizhedath, Micael Karlberg, Professor Jarka Glassey

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Abstract

© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, WeinheimMonoclonal antibodies (mAbs) constitute a rapidly growing biopharmaceutical sector. However, their growth is impeded by developability issues such as polyspecificity and lack of solubility, which leads to attrition as well as manufacturing failures. In this study a multitool hybrid quantitative structure–activity relationship (QSAR) model development framework is described. This framework uses four novel datasets derived from the primary sequences of IgG1-κ-humanized mAbs with varying degrees of resolutions. Unsupervised pattern recognition is first performed on the descriptor sets to visualize any intrinsic property-based clustering, followed by regression of descriptors against cross-interaction chromatography (CIC) retention times. Model optimization is performed via unsupervised variable reduction followed by supervised variable selection. Finally, the models and datasets are benchmarked based on the regression model performance metrics such as R2, Q2, and RMSE. The results show that datasets containing localized descriptors rather than averaged value over the entire protein have better predictive performance of CIC retention behavior with R2 > 0.8 and RMSE < 0.3. Furthermore, the results indicate the physicochemical, electronic, and topological properties of hypervariable regions of antibodies that contribute most to the CIC retention times. The results of these studies could contribute to early-stage screening and better design of mAbs.


Publication metadata

Author(s): Kizhedath A, Karlberg M, Glassey J

Publication type: Article

Publication status: Published

Journal: Biotechnology Journal

Year: 2019

Volume: 14

Issue: 8

Print publication date: 01/08/2019

Online publication date: 17/04/2019

Acceptance date: 27/02/2019

ISSN (print): 1860-6768

ISSN (electronic): 1860-7314

Publisher: Wiley-VCH Verlag

URL: https://doi.org/10.1002/biot.201800696

DOI: 10.1002/biot.201800696

PubMed id: 30810283


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