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Cold-modulated small proteins abundance in winter triticale (x Triticosecale, Wittm.) seedlings tolerant to the pink snow mould (Microdochium nivale, Samuels and Hallett) infection

Lookup NU author(s): Dr Kaveh Emami

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Abstract

Two winter triticale (x Triticosecale Wittmack) model cultivars: Hewo (tolerant to pink snow mould) and Magnat (sensitive) were used to test the effect of cold-hardening (4 weeks at 4°C) on soluble ≤50 kDa protein profiles of the seedling leaves. The presence and abundance of individual proteins were analysed via two-dimensional gel electrophoresis (2-DE) and Surface-Enhanced Laser Desorption/Ionization Time-of-Flight (SELDI-TOF). Up to now, no proteomics analysis of triticale response to hardening has been performed. Thus, the present paper is the first in the series describing the obtained results. In our experiments, the exposure to the low temperature-induced only quantitative changes in the leaves of both cultivars, causing either an increase or decrease of 4-50 kDa protein abundance. Among proteins which were cold-accumulated in cv. Hewo's leaves, we identified two thioredoxin peroxidases (chloroplastic thiol-specific antioxidant proteins) as well as mitochondrial- β-ATP synthase subunit and ADP-binding resistance protein. On the contrary, in hardened seedlings of this genotype, we observed the decreased level of chloroplastic RuBisCO small subunit PW9 and epidermal peroxidase 10. Simultaneous SELDI-TOF analysis revealed several low mass proteins better represented in cold-hardened plants of tolerant genotype in comparison to the sensitive one and the impact of both genotype and temperature on their level. Based on those results, we suggest that indicated proteins might be potential candidates for molecular markers of cold-induced snow mould resistance of winter triticale and their role is worth to be investigated in the further inoculation experiments.


Publication metadata

Author(s): Golebiowska GJ, Bonar E, Emami K, Wedzony M

Publication type: Article

Publication status: Published

Journal: Acta biochimica Polonica

Year: 2019

Volume: 66

Issue: 3

Pages: 343-350

Print publication date: 17/09/2019

Online publication date: 10/09/2019

Acceptance date: 22/07/2019

Date deposited: 02/10/2019

ISSN (print): 0001-527X

ISSN (electronic): 1734-154X

Publisher: Polskie Towarzystwo Biochemiczne,Polish Biochemical Society

URL: https://doi.org/10.18388/abp.2019_2804

DOI: 10.18388/abp.2019_2804

PubMed id: 31509370


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