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Lookup NU author(s): Dr Rachel Botting, Dr Dorin-Mirel Popescu, Dr Roser Vento-Tormo, Emily Stephenson, Dr Kile GreenORCiD, Professor Andrew FilbyORCiD, Dr Steven LisgoORCiD, Emerita Professor Susan Lindsay, Professor Muzlifah Haniffa
This is the authors' accepted manuscript of an article that has been published in its final definitive form by American Association for the Advancement of Science, 2019.
For re-use rights please refer to the publisher's terms and conditions.
Copyright © 2019 The Authors.Tissue-resident immune cells are important for organ homeostasis and defense. The epithelium may contribute to these functions directly or by cross-talk with immune cells. We used single-cell RNA sequencing to resolve the spatiotemporal immune topology of the human kidney. We reveal anatomically defined expression patterns of immune genes within the epithelial compartment, with antimicrobial peptide transcripts evident in pelvic epithelium in the mature, but not fetal, kidney. A network of tissue-resident myeloid and lymphoid immune cells was evident in both fetal and mature kidney, with postnatal acquisition of transcriptional programs that promote infection-defense capabilities. Epithelial-immune cross-talk orchestrated localization of antibacterial macrophages and neutrophils to the regions of the kidney most susceptible to infection. Overall, our study provides a global overview of how the immune landscape of the human kidney is zonated to counter the dominant immunological challenge.
Author(s): Stewart BJ, Ferdinand JR, Young MD, Mitchell TJ, Loudon KW, Riding AM, Richoz N, Frazer GL, Staniforth JUL, Braga FAV, Botting RA, Popescu D-M, Vento-Tormo R, Stephenson E, Cagan A, Farndon SJ, Polanski K, Efremova M, Green K, Velasco-Herrera MDC, Guzzo C, Collord G, Mamanova L, Aho T, Armitage JN, Riddick ACP, Mushtaq I, Farrell S, Rampling D, Nicholson J, Filby A, Burge J, Lisgo S, Lindsay S, Bajenoff M, Warren AY, Stewart GD, Sebire N, Coleman N, Haniffa M, Teichmann SA, Behjati S, Clatworthy MR
Publication type: Article
Publication status: Published
Journal: Science
Year: 2019
Volume: 365
Issue: 6460
Pages: 1461-1466
Print publication date: 27/09/2019
Online publication date: 04/09/2019
Acceptance date: 04/09/2019
Date deposited: 28/10/2019
ISSN (print): 0036-8075
ISSN (electronic): 1095-9203
Publisher: American Association for the Advancement of Science
URL: https://doi.org/10.1126/science.aat5031
DOI: 10.1126/science.aat5031
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