Toggle Main Menu Toggle Search

Open Access padlockePrints

Dysregulation of Mitochondrial Ca2+ Uptake and Sarcolemma Repair Underlie Muscle Weakness and Wasting in Patients and Mice Lacking MICU1

Lookup NU author(s): Professor Rita Horvath


Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


© 2019 The AuthorsMuscle function is regulated by Ca2+, which mediates excitation-contraction coupling, energy metabolism, adaptation to exercise, and sarcolemmal repair. Several of these actions rely on Ca2+ delivery to the mitochondrial matrix via the mitochondrial Ca2+ uniporter, the pore of which is formed by mitochondrial calcium uniporter (MCU). MCU's gatekeeping and cooperative activation are controlled by MICU1. Loss-of-protein mutation in MICU1 causes a neuromuscular disease. To determine the mechanisms underlying the muscle impairments, we used MICU1 patient cells and skeletal muscle-specific MICU1 knockout mice. Both these models show a lower threshold for MCU-mediated Ca2+ uptake. Lack of MICU1 is associated with impaired mitochondrial Ca2+ uptake during excitation-contraction, aerobic metabolism impairment, muscle weakness, fatigue, and myofiber damage during physical activity. MICU1 deficit compromises mitochondrial Ca2+ uptake during sarcolemmal injury, which causes ineffective repair of the damaged myofibers. Thus, dysregulation of mitochondrial Ca2+ uptake hampers myofiber contractile function, likely through energy metabolism and membrane repair.© 2019 The AuthorsDebattisti et al. report that skeletal muscle-specific loss of mitochondrial Ca2+ uptake 1 (MICU1) in mouse impairs mitochondrial calcium signaling, energy metabolism, and membrane repair, leading to muscle weakness, fatigue, myofiber damage, and high CK levels, recapitulating the muscle symptoms of MICU1 loss in patients.

Publication metadata

Author(s): Debattisti V, Horn A, Singh R, Seifert EL, Hogarth MW, Mazala DA, Huang KT, Horvath R, Jaiswal JK, Hajnoczky G

Publication type: Article

Publication status: Published

Journal: Cell Reports

Year: 2019

Volume: 29

Issue: 5

Pages: 1274-1286.e6

Print publication date: 29/10/2019

Online publication date: 30/10/2019

Acceptance date: 20/09/2019

Date deposited: 04/11/2019

ISSN (print): 2211-1247

ISSN (electronic): 2211-1247

Publisher: Elsevier B.V.


DOI: 10.1016/j.celrep.2019.09.063


Altmetrics provided by Altmetric


Find at Newcastle University icon    Link to this publication