Browse by author
Lookup NU author(s): Dr Alan Cartmell, Dr Jose Munoz Munoz, Dr Jonathan Briggs, Dr Didier Ndeh, Dr Elisabeth Lowe, Dr Arnaud Basle, Dr Tiaan Heunis, Dr Joseph Gray, Dr Aurore Labourel, Professor Matthias TrostORCiD, Emeritus Professor Harry Gilbert
Full text for this publication is not currently held within this repository. Alternative links are provided below where available.
© 2019, The Author(s), under exclusive licence to Springer Nature Limited. In the version of this Article originally published, we reported the protein BT3679 to be an a-l-arabinofuranosidase. Recent studies have shown that BT3679 has no demonstrable catalytic activity and thus remains a protein of unknown function. This error was detected in the laboratory of Gideon Davies, at the University of York, who was attempting to obtain the crystal structure of BT3679 in complex with appropriate ligands. His group generated BT3679 independently of our work, and showed that the protein displayed no a-l-arabinofuranosidase activity. Once alerted to this possibility, we explored the plasmid population in the master stock of BT3679. Transforming the stock into Escherichia coli and sequencing the plasmid in five single colonies showed that the stock contained two plasmids: BT3679 and BT0348. The BT0348 plasmid was previously shown to encode a GH51 a-l-arabinofuranosidase (Nat. Microbiol. 3, 210–119; 2017). Thus, the reported a-l-arabinofuranosidase activity comprised BT0348, but not BT3679. This was confirmed by the Davies laboratory when they analysed a sample of the protein, purified by immobilized metal affinity chromatography from E. coli containing the contaminated BT3679 plasmid preparation; they showed that an a-l-arabinofuranosidase probe labelled a 51 kDa protein corresponding to the size of the BT0348 plasmid, but not to the 47 kDa BT3679 band. The Article has thus been amended as follows: (1) Information regarding BT3679 has been removed from the degradative model shown in Fig. 1a,b. Supplementary Fig. 7b,c sub-figures have been removed, and panels referring to BT3679 have been removed from Supplementary Fig. 8; the respective captions have been amended to reflect such changes. Information regarding BT3679 has also been removed from Supplementary Table 2. (2) The designation of BT3679 as a protein of no detectable activity has been stated in Fig. 1c and Supplementary Table 1. (3) Other text referring to BT3679 has been deleted from the Article.
Author(s): Cartmell A, Munoz-Munoz J, Briggs JA, Ndeh DA, Lowe EC, Basle A, Terrapon N, Stott K, Heunis T, Gray J, Yu L, Dupree P, Fernandes PZ, Shah S, Williams SJ, Labourel A, Trost M, Henrissat B, Gilbert HJ
Publication type: Note
Publication status: Published
Journal: Nature Microbiology
Year: 2019
Volume: 4
Issue: 11
Pages: 2021-2023
Online publication date: 22/10/2019
Acceptance date: 02/04/2019
ISSN (electronic): 2058-5276
Publisher: Nature Publishing Group
URL: https://doi.org/10.1038/s41564-018-0258-8
DOI: 10.1038/s41564-019-0584-5
PubMed id: 31541200
