Lookup NU author(s): Dr Diana Papini
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
Timely and precise control of Aurora B kinase, the chromosomal passenger complex (CPC) catalytic subunit, is essential for accurate chromosome segregation and cytokinesis. Post-translational modifications of CPC subunits are directly involved in controlling Aurora B activity. Here, we identified a highly conserved acidic STD-rich motif of INCENP that is phosphorylated during mitosis in vivo and by Plk1 in vitro and is involved in controlling Aurora B activity. By using an INCENP conditional-knockout cell line, we show that impairing the phosphorylation status of this region disrupts chromosome congression and induces cytokinesis failure. In contrast, mimicking constitutive phosphorylation not only rescues cytokinesis but also induces ectopic furrows and contractile ring formation in a Plk1- and ROCK1-dependent manner independent of cell cycle and microtubule status. Our experiments identify the phospho-regulation of the INCENP STD motif as a novel mechanism that is key for chromosome alignment and cytokinesis.This article has an associated First Person interview with the first author of the paper.
Author(s): Papini D, Fant X, Ogawa H, Desban N, Samejima K, Feizbakhsh O, Askin B, Ly T, Earnshaw WC, Ruchaud S
Publication type: Article
Publication status: Published
Journal: Journal of Cell Science
Print publication date: 06/11/2019
Online publication date: 10/10/2019
Acceptance date: 27/09/2019
Date deposited: 21/11/2019
ISSN (print): 0021-9533
ISSN (electronic): 1477-9137
Publisher: Company of Biologists
PubMed id: 31601613
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