Toggle Main Menu Toggle Search

Open Access padlockePrints

Cell cycle-independent furrowing triggered by phosphomimetic mutations of the INCENP STD motif requires Plk1

Lookup NU author(s): Dr Diana Papini

Downloads


Licence

This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).


Abstract

Timely and precise control of Aurora B kinase, the chromosomal passenger complex (CPC) catalytic subunit, is essential for accurate chromosome segregation and cytokinesis. Post-translational modifications of CPC subunits are directly involved in controlling Aurora B activity. Here, we identified a highly conserved acidic STD-rich motif of INCENP that is phosphorylated during mitosis in vivo and by Plk1 in vitro and is involved in controlling Aurora B activity. By using an INCENP conditional-knockout cell line, we show that impairing the phosphorylation status of this region disrupts chromosome congression and induces cytokinesis failure. In contrast, mimicking constitutive phosphorylation not only rescues cytokinesis but also induces ectopic furrows and contractile ring formation in a Plk1- and ROCK1-dependent manner independent of cell cycle and microtubule status. Our experiments identify the phospho-regulation of the INCENP STD motif as a novel mechanism that is key for chromosome alignment and cytokinesis.This article has an associated First Person interview with the first author of the paper.


Publication metadata

Author(s): Papini D, Fant X, Ogawa H, Desban N, Samejima K, Feizbakhsh O, Askin B, Ly T, Earnshaw WC, Ruchaud S

Publication type: Article

Publication status: Published

Journal: Journal of Cell Science

Year: 2019

Volume: 132

Issue: 21

Print publication date: 06/11/2019

Online publication date: 10/10/2019

Acceptance date: 27/09/2019

Date deposited: 21/11/2019

ISSN (print): 0021-9533

ISSN (electronic): 1477-9137

Publisher: Company of Biologists

URL: https://doi.org/10.1242/jcs.234401

DOI: 10.1242/jcs.234401

PubMed id: 31601613


Altmetrics

Altmetrics provided by Altmetric


Actions

Find at Newcastle University icon    Link to this publication


Share