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Lookup NU author(s): Elena Mancuso,
Dr Piergiorgio Gentile
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
The use of antibiotics has been the cornerstone to prevent bacterial infections; however, the emergency of antibiotic-resistant bacteria is still an open challenge. This work aimed to develop a delivery system for treating soft tissue infections for: (1) reducing the released antimicrobial amount, preventing drug-related systemic side effects; (2) rediscovering the beneficial effects of naturally derived agents; and (3) preserving the substrate functional properties. For the first time, Manuka honey (MH) was proposed as polyelectrolyte within the layer-by-layer assembly. Biomimetic electrospun poly(εcaprolactone) meshes were treated via layer-by-layer assembly to obtain a multilayered nanocoating, consisting of MH as polyanion and poly-(allylamine-hydrochloride) as polycation. Physicochemical characterization demonstrated the successful nanocoating formation. Different cell lines (human immortalized and primary skin fibroblasts, and primary endothelial cells) confirmed positively the membranes cytocompatibility, while bacterial tests using Gram-negative and Gram-positive bacteria demonstrated that the antimicrobial MH activity was dependent on the concentration used and strains tested.
Author(s): Mancuso E, Tonda-Turo C, Ceresa C, Pensabene V, Connell SD, Fracchia L, Gentile P
Publication type: Article
Publication status: Published
Journal: Frontiers in Bioengineering and Biotechnology
Print publication date: 04/12/2019
Online publication date: 04/12/2019
Acceptance date: 06/11/2019
Date deposited: 13/12/2019
ISSN (electronic): 2296-4185
Publisher: Frontiers Research Foundation
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