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Extracellular DNA Provides Structural Integrity to a Micrococcus luteus Biofilm

Lookup NU author(s): Dr Ana Lorena Morales Garcia, Professor Mark GeogheganORCiD

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This is the authors' accepted manuscript of an article that has been published in its final definitive form by American Chemical Society, 2019.

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Abstract

© 2019 American Chemical Society.Force spectroscopy was used to show that extracellular DNA (eDNA) has a pre-eminent structural role in a biofilm. The adhesive behavior of extracellular polymeric substances to poly(ethylene terephthalate), a model hydrophobic surface, was measured in response to their degradation by hydrolytic enzymes known for their biofilm dispersion potential: DNaseI, protease, cellulase, and mannanase. Only treatment with DNaseI significantly decreased the adhesive force of the model bacterium Micrococcus luteus with the surface, and furthermore this treatment almost completely eliminated any components of the biofilm maintaining the adhesion, establishing a key structural role for eDNA.

Publication metadata

Author(s): Blakeman JT, Morales-Garcia AL, Mukherjee J, Gori K, Hayward AS, Lant NJ, Geoghegan M

Publication type: Article

Publication status: Published

Journal: Langmuir

Year: 2019

Volume: 35

Issue: 19

Pages: 6468-6475

Online publication date: 17/04/2019

Acceptance date: 02/04/2019

Date deposited: 10/11/2020

ISSN (print): 0743-7463

ISSN (electronic): 1520-5827

Publisher: American Chemical Society

URL: https://doi.org/10.1021/acs.langmuir.9b00297

DOI: 10.1021/acs.langmuir.9b00297

PubMed id: 30995049

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