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Candida albicans biofilm heterogeneity does not influence denture stomatitis but strongly influences denture cleansing capacity

Lookup NU author(s): Dr Christopher Nile

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This is the authors' accepted manuscript of an article that has been published in its final definitive form by Microbiology Society, 2017.

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Abstract

© 2017 The Authors.Approximately 20% of the UK population wear some form of denture prosthesis, resulting in denture stomatitis in half of these individuals. Candida albicans is primarily attributed as the causative agent, due to its biofilm -forming ability. Recently, there has been increasing evidence of C. albicans biofilm heterogeneity and the negative impact it can have clinically; however, this phenomenon has yet to be studied in relation to denture isolates. The aims of this study were to evaluate C. albicans biofilm formation of clinical denture isolates in a denture environment and to assess antimicrobial activity of common denture cleansers against these tenacious communities. C. albicans isolated from dentures of healthy and diseased individuals was quantified using real-time PCR and biofilm biomass assessed using crystal violet. Biofilm development on the denture substratum poly(methyl methacrylate), Molloplast B and Ufi-gel was determined. Biofilm formation was assessed using metabolic and biomass stains, following treatment with denture hygiene products. Although C. albicans was detected in greater quantities in diseased individuals, it was not associated with increased biofilm biomass. Denture substrata were shown to influence biofilm biomass, with poly(methyl methacrylate) providing the most suitable environment for C. albicans to reside. Of all denture hygiene products tested, Milton had the most effective antimicrobial activity, reducing biofilm biomass and viability the greatest. Overall, our results highlight the complex nature of denturerelated disease, and disease development cannot always be attributed to a sole cause. It is the distinct combination of various factors that ultimately determines the pathogenic outcome.


Publication metadata

Author(s): O'Donnell LE, Alalwan HKA, Kean R, Calvert G, Nile CJ, Lappin DF, Robertson D, Williams C, Ramage G, Sherry L

Publication type: Article

Publication status: Published

Journal: Journal of Medical Microbiology

Year: 2017

Volume: 66

Issue: 1

Pages: 54-60

Online publication date: 01/01/2017

Acceptance date: 28/12/2017

Date deposited: 24/02/2020

ISSN (print): 0022-2615

ISSN (electronic): 1473-5644

Publisher: Microbiology Society

URL: https://doi.org/10.1099/jmm.0.000419

DOI: 10.1099/jmm.0.000419

PubMed id: 28032543


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