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Lookup NU author(s): Dr Adam WollmanORCiD
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
© 2016 IOP Publishing Ltd. Staphylococcus aureus is an important pathogen, giving rise to antimicrobial resistance in cell strains such as Methicillin Resistant S. aureus (MRSA). Here we report an image analysis framework for automated detection and image segmentation of cells in S. aureus cell clusters, and explicit identification of their cell division planes. We use a new combination of several existing analytical tools of image analysis to detect cellular and subcellular morphological features relevant to cell division from millisecond time scale sampled images of live pathogens at a detection precision of single molecules. We demonstrate this approach using a fluorescent reporter GFP fused to the protein EzrA that localises to a mid-cell plane during division and is involved in regulation of cell size and division. This image analysis framework presents a valuable platform from which to study candidate new antimicrobials which target the cell division machinery, but may also have more general application in detecting morphologically complex structures of fluorescently labelled proteins present in clusters of other types of cells.
Author(s): Wollman AJM, Miller H, Foster S, Leake MC
Publication type: Article
Publication status: Published
Journal: Physical Biology
Year: 2016
Volume: 13
Issue: 5
Online publication date: 17/10/2016
Acceptance date: 22/08/2016
Date deposited: 10/02/2020
ISSN (print): 1478-3967
ISSN (electronic): 1478-3975
Publisher: Institute of Physics Publishing
URL: https://doi.org/10.1088/1478-3975/13/5/055002
DOI: 10.1088/1478-3975/13/5/055002
PubMed id: 27749270
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