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Transcriptome analysis of Pseudomonas aeruginosa PAO1 grown at both body and elevated temperatures

Lookup NU author(s): Dr Chien-Yi ChangORCiD

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This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).


Abstract

© 2016 Chan et al.Functional genomics research can give us valuable insights into bacterial gene function. RNA Sequencing (RNA-seq) can generate information on transcript abundance in bacteria following abiotic stress treatments. In this study, we used the RNA-seq technique to study the transcriptomes of the opportunistic nosocomial pathogen Pseudomonas aeruginosa PAO1 following heat shock. Samples were grown at both the human body temperature (37 ° C) and an arbitrarily-selected temperature of 46 ° C. In this work using RNA-seq, we identified 133 genes that are differentially expressed at 46 ° C compared to the human body temperature. Our work identifies some key P. aeruginosa PAO1 genes whose products have importance in both environmental adaptation as well as in vivo infection in febrile hosts. More importantly, our transcriptomic results show that many genes are only expressed when subjected to heat shock. Because the RNA-seq can generate high throughput gene expression profiles, our work reveals many unanticipated genes with further work to be done exploring such genes products.


Publication metadata

Author(s): Chan K-G, Priya K, Chang C-Y, Abdul Rahman AY, Tee KK, Yin W-F

Publication type: Article

Publication status: Published

Journal: PeerJ

Year: 2016

Volume: 2016

Issue: 7

Online publication date: 19/07/2016

Acceptance date: 15/06/2016

Date deposited: 25/02/2020

ISSN (electronic): 2167-8359

Publisher: PeerJ Ltd

URL: https://doi.org/10.7717/peerj.2223

DOI: 10.7717/peerj.2223

PubMed id: 27547539


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