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Lookup NU author(s): Sabine Gretenkord,
Dr Bas Olthof,
Professor Adrian Rees,
Dr Sasha Gartside,
Dr Fiona LeBeau
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
The role of dopamine in regulating sleep-state transitions during both natural sleep and under anaesthesia is still unclear. Recording in vivo in the rat mPFC under urethane anesthesia we have observed predominantly slow wave activity (SWA) of <1 Hz in the local field potential interrupted by occasional spontaneous transitions to a low-amplitude-fast (LAF) pattern of activity. During periods of SWA, transitions to LAF activity could be rapidly and consistently evoked by electrical stimulation of the ventral tegmental area (VTA). Both the spontaneous, and VTA stimulation-evoked LAF activity, consisted of fast oscillations similar to those seen in the rapid eye movement (REM)-like sleep state. Spontaneous and VTA stimulation evoked LAF activity occurred simultaneously along the dorso-ventral extent of all mPFC subregions. Evoked LAF activity depended on VTA stimulation current, and could be elicited using either regular (25 - 50 Hz) or burst stimulation patterns, and was reproducible upon repeated stimulations. Simultaneous single unit recordings showed that during SWA presumed pyramidal cells fired phasically, and almost exclusively on the Up-state, while during both spontaneous and VTA-evoked LAF activity they fired tonically. The transition to LAF activity evoked by VTA stimulation depended on dopamine D1-like receptor activation as it was almost completely blocked by systemic administration of the D1-like receptor antagonist SCH23390. Overall our data demonstrate that activation of dopamine D1-like receptors in the mPFC is important for regulating sleep-like state transitions.
Author(s): Gretenkord S, Olthof BMJ, Stylianou M, Rees A, Gartside SE, LeBeau FEN
Publication type: Article
Publication status: Published
Journal: European Journal of Neuroscience
Issue: ePub ahead of Print
Online publication date: 31/12/2019
Acceptance date: 27/12/2019
Date deposited: 12/03/2020
ISSN (print): 0953-816X
ISSN (electronic): 1460-9568
Publisher: Wiley-Blackwell Publishing Ltd.
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