Lookup NU author(s): Dr Christopher Stewart
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© 2020, Springer Science+Business Media, LLC, part of Springer Nature.The highly parallel nature of sequencing by synthesis (SBS) allows millions of amplicons to be sequenced simultaneously, which has led to enormous interest in the investigation of bacterial communities (often referred to as the microbiota). In this protocol, we describe a method for the ‘universal’ amplification of the v4 region of the bacterial 16S rRNA gene from genomic DNA and prepare these amplicons so that they can be sequenced using the MiSeq system (Illumina). The protocol provides instruction on sequencing of 188 genomic DNA samples plus PCR positive and negative controls, which can be applied to any sample type where bacterial DNA may be of interest.
Author(s): Nelson A, Stewart CJ
Publication type: Book Chapter
Publication status: Published
Book Title: Methods in Molecular Biology
Online publication date: 09/03/2020
Acceptance date: 02/04/2016
Series Title: Methods in Molecular Biology
Publisher: Humana Press Inc.
PubMed id: 32147795
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