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Structure-activity Analysis of CJ-15,801 Analogues That Interact With Plasmodium Falciparum Pantothenate Kinase and Inhibit Parasite Proliferation

Lookup NU author(s): Dr Stephen Hobson

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Abstract

Survival of the human malaria parasite Plasmodium falciparum is dependent on pantothenate (vitamin B₅), a precursor of the fundamental enzyme cofactor coenzyme A. CJ-15,801, an enamide analogue of pantothenate isolated from the fungus Seimatosporium sp. CL28611, was previously shown to inhibit P. falciparum proliferation in vitro by targeting pantothenate utilization. To inform the design of next generation analogues, we set out to synthesize and test a series of synthetic enamide-bearing pantothenate analogues. We demonstrate that conservation of the R-pantoyl moiety and the trans-substituted double bond of CJ-15,801 is important for the selective, on-target antiplasmodial effect, while replacement of the carboxyl group is permitted, and, in one case, favored. Additionally, we show that the antiplasmodial potency of CJ-15,801 analogues that retain the R-pantoyl and trans-substituted enamide moieties correlates with inhibition of P. falciparum pantothenate kinase (PfPanK)-catalyzed pantothenate phosphorylation, implicating the interaction with PfPanK as a key determinant of antiplasmodial activity.

Publication metadata

Author(s): Spry C, Sewell AL, Hering Y, Villa MVJ, Weber J, Hobson SJ, Harnor SJ, Gul S, Marquez R, Saliba KJ

Publication type: Article

Publication status: Published

Journal: European Journal of Medicinal Chemistry

Year: 2018

Volume: 143

Pages: 1139-1147

Print publication date: 01/01/2018

Online publication date: 25/08/2017

Acceptance date: 31/05/2017

ISSN (print): 0223-5234

ISSN (electronic): 1768-3254

Publisher: Elsevier

URL: https://doi.org/10.1016/j.ejmech.2017.08.050

DOI: 10.1016/j.ejmech.2017.08.050

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