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Lookup NU author(s): Joseph Dudman,
Dr Ana Ferreira-Duarte,
Dr Piergiorgio Gentile,
Dr Xiao Wang,
Dr Matthew Benning,
Professor Kenneth Dalgarno
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
Drop-on-demand (DoD) inkjet printing has been explored for a range of applications, including those to selectively deposit cellular material, due to the high accuracy and scalability of such systems when compared with alternative bioprinting techniques. Despite this, there remains considerable limitations when handling cell suspensions due to the agglomeration and sedimentation of cells during printing, leading to a deterioration in jetting performance. The objective of this work was to design and assess the effectiveness of a custom agitation system to maintain cellular dispersion within the ink reservoir during printing. The cell printing performance of an inkjet printer was assessed with and without the use of a custom agitation system, with biological characterisation performed to characterise the impact of the agitator on cellular viability and function. Cell printing performance was retained over a 2-hour printing period when incorporating an agitated reservoir, with a gradual reduction in performance observed under a non-agitated configuration. Cell assays indicated that the agitation process did not significantly affect the viability, metabolic activity or morphology of the mesenchymal stromal cell or chondrocyte cell types. This study therefore provides a new methodology to increase process reliability within DoD printing platforms when jetting cellularised material.
Author(s): Dudman J, Ferreira AM, Gentile P, Wang XN, Ribeiro R, Benning M, Dalgarno K
Publication type: Article
Publication status: Published
Issue: ePub ahead of Print
Online publication date: 06/07/2020
Acceptance date: 06/07/2020
Date deposited: 06/07/2020
ISSN (print): 1758-5082
ISSN (electronic): 1758-5090
Publisher: Institute of Physics Publishing Ltd.
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