Browse by author
Lookup NU author(s): Dr Owen Davies
Full text for this publication is not currently held within this repository. Alternative links are provided below where available.
© 2020 Sánchez Rodríguez et al.The conventional approach in molecular replacement is the use of a related structure as a search model. However, this is not always possible as the availability of such structures can be scarce for poorly characterized families of proteins. In these cases, alternative approaches can be explored, such as the use of small ideal fragments that share high, albeit local, structural similarity with the unknown protein. Earlier versions of AMPLE enabled the trialling of a library of ideal helices, which worked well for largely helical proteins at suitable resolutions. Here, the performance of libraries of helical ensembles created by clustering helical segments is explored. The impacts of different B-factor treatments and different degrees of structural heterogeneity are explored. A 30% increase in the number of solutions obtained by AMPLE was observed when using this new set of ensembles compared with the performance with ideal helices. The boost in performance was notable across three different fold classes: Transmembrane, globular and coiled-coil structures. Furthermore, the increased effectiveness of these ensembles was coupled to a reduction in the time required by AMPLE to reach a solution. AMPLE users can now take full advantage of this new library of search models by activating the 'helical ensembles' mode.
Author(s): Rodriguez FS, Simpkin AJ, Davies OR, Keegan RM, Rigden DJ
Publication type: Article
Publication status: Published
Journal: Acta Crystallographica Section D: Structural Biology
Print publication date: 01/10/2020
Online publication date: 15/10/2020
Acceptance date: 18/08/2020
ISSN (electronic): 2059-7983
Publisher: International Union of Crystallography
PubMed id: 33021498
Altmetrics provided by Altmetric