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Lookup NU author(s): Alex Ling,
Dr Yuen Ling Ng,
Professor Anil Wipat
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
© Copyright © 2020 Ling, Su, Lua, Poh, Ng, Wipat and Gan.Boosting the production of recombinant therapeutic antibodies is crucial in both academic and industry settings. In this work, we investigated the usage of varying signal peptides by antibody V-genes and their roles in recombinant transient production, systematically comparing myeloma and the native signal peptides of both heavy and light chains in 168 antibody permutation variants. We found that amino acids count and types (essential or non-essential) were important factors in a logistic regression equation model for predicting transient co-transfection protein production rates. Deeper analysis revealed that the culture media were often incomplete and that the supplementation of essential amino acids can improve the recombinant protein yield. While these findings are derived from transient HEK293 expression, they also provide insights to the usage of the large repertoire of antibody signal peptides, where by varying the number of specific amino acids in the signal peptides attached to the variable regions, bottlenecks in amino acid availability can be mitigated.
Author(s): Ling W-L, Su CT-T, Lua W-H, Poh J-J, Ng Y-L, Wipat A, Gan SK-E
Publication type: Article
Publication status: Published
Journal: Frontiers in Immunology
Print publication date: 01/12/2020
Online publication date: 07/12/2020
Acceptance date: 05/11/2020
Date deposited: 03/02/2021
ISSN (electronic): 1664-3224
Publisher: Frontiers Media S.A.
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