3D-printed Soft Hydrogels for Cell Encapsulation

Scalzone, A; Tonda-Turo, C; Ferreira, AM; Gentile, P

doi:10.1039/9781839161124-00594

3D-printed Soft Hydrogels for Cell Encapsulation

Lookup NU author(s): Dr Annachiara Scalzone ORCiD, Dr Ana Ferreira-Duarte ORCiD, Dr Piergiorgio Gentile ORCiD

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Abstract

© The Royal Society of Chemistry 2021.Recent advances in 3D-printing with a combination of cell biology with materials science allowed the manufacturing of cell-encapsulated biomaterials to be used in tissue engineering. The main component of these bioprinted constructs is a bioink that requires its characteristics to be considered and tuned throughout the selection process. In this chapter, the focus is on the physico-chemical and biological requirements of the hydrogels being considered as bioinks with a deep investigation on the different crosslinking mechanisms that allow a stable hydrogel to be created during the bioprinting process. This chapter also describes the current focus areas for cell-encapsulated bioprinting highlighting the recent progress and limitations of the bioprinting to date, with a critical perspective on the future outlook.