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Lookup NU author(s): Dr Jordan CuffORCiD
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Punjab urial (Ovis vignei punjabiensis) is a wild sheep of Pakistan, considered a vulnerable species by IUCN. Major threats to urial populations include habitat loss and poaching, causing severe declines in its population. Nematode infections may also compromise urial survival, but little is known about Punjab urial gastrointestinal nematodes. In this study, a novel DNA barcoding approach was developed using ITS-I as a target region, with a primer pair designed to amplify frequently reported nematode species for small ruminants. The novel primer pair was validated in silico and in vitro and subsequently used to determine the presence of nematodes in Punjab urial samples from Kala Bagh Game Reserve, District Mianwali (Pakistan). DNA barcoding revealed a higher prevalence of Haemonchus contortus (73.91%), Trichuris ovis (16.30%) and Trichostrongylus axei (3.26%) in Punjab urial. This study demonstrates that the novel DNA barcoding approach is a robust tool to detect nematode parasites from faecal samples of Punjab urial. This method can be used to detect nematode infections in wild and domestic hosts for surveillance and population conservation.
Author(s): Bajwa AA, Cuff JP, Imran M, Islam S, Mansha R, Ashraf K, Khan A, Rashid MI, Zahoor MY, Khan WA, Rehman HU, Nadeem A, Orozco-terWengel P, Shehzad W
Publication type: Article
Publication status: Published
Journal: European Journal of Wildlife Research
Year: 2019
Volume: 65
Online publication date: 13/07/2019
Acceptance date: 01/07/2019
ISSN (print): 1612-4642
ISSN (electronic): 1439-0574
Publisher: Springer Nature
URL: https://doi.org/10.1007/s10344-019-1298-y
DOI: 10.1007/s10344-019-1298-y
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