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Lookup NU author(s): Rhianna RowlandORCiD
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Plant polysaccharides represent a virtually unlimited feedstock for the generation of biofuels and other commodities. However, the extraordinary recalcitrance of plant polysaccharides toward breakdown necessitates a continued search for enzymes that degrade these materials efficiently under defined conditions. Activity-based protein profiling provides a route for the functional discovery of such enzymes in complex mixtures and under industrially relevant conditions. Here, we show the detection and identification of β-xylosidases and endo-β-1,4-xylanases in the secretomes of Aspergillus niger, by the use of chemical probes inspired by the β-glucosidase inhibitor cyclophellitol. Furthermore, we demonstrate the use of these activity-based probes (ABPs) to assess enzyme–substrate specificities, thermal stabilities, and other biotechnologically relevant parameters. Our experiments highlight the utility of ABPs as promising tools for the discovery of relevant enzymes useful for biomass breakdown.
Author(s): Schroder SP, de Boer C, McGregor NGS, Rowland RJ, Moroz O, Blagova E, Reijngoud J, Arentshorst M, Osborn D, Morant MD, Abbate E, Stringer MA, Krogh KBRM, Raich L, Rovira C, Berrin J, van Wezel GP, Ram AFJ, Florea BI, van der Marel GA, Codée JDC, Wilson KS, Wu L, Davies GD, Overkleeft HS
Publication type: Article
Publication status: Published
Journal: ACS Central Science
Year: 2019
Volume: 5
Issue: 6
Pages: 1067-1078
Print publication date: 26/06/2019
Online publication date: 24/05/2019
Acceptance date: 24/04/2019
ISSN (electronic): 2374-7951
Publisher: American Chemical Society
URL: https://doi.org/10.1021/acscentsci.9b00221
DOI: 10.1021/acscentsci.9b00221
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