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Lookup NU author(s): Dr Sharon Velasquez OrtaORCiD, Dr Leonardo Rios Solis
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
© 2022 The Author(s). In this study, organic acids were demonstrated as a promising carbon source for bisabolene production by the non-conventional yeast, Rhodosporidium toruloides, at microscale with a maximum titre of 1055 ± 7 mg/L. A 125-fold scale-up of the optimal process, enhanced bisabolene titres 2.5-fold to 2606 mg/L. Implementation of a pH controlled organic acid feeding strategy at this scale lead to a further threefold improvement in bisabolene titre to 7758 mg/L, the highest reported microbial titre. Finally, a proof-of-concept sequential bioreactor approach was investigated. Firstly, the cellulolytic bacterium Ruminococcus flavefaciens was employed to ferment cellulose, yielding 4.2 g/L of organic acids. R. toruloides was subsequently cultivated in the resulting supernatant, producing 318 ± 22 mg/L of bisabolene. This highlights the feasibility of a sequential bioprocess for the bioconversion of cellulose, into biojet fuel candidates. Future work will focus on enhancing organic acid yields and the use of real lignocellulosic feedstocks to further enhance bisabolene production.
Author(s): Walls LE, Otoupal P, Ledesma-Amaro R, Velasquez-Orta SB, Gladden JM, Rios-Solis L
Publication type: Article
Publication status: Published
Journal: Bioresource Technology
Year: 2023
Volume: 368
Print publication date: 01/01/2023
Online publication date: 05/11/2022
Acceptance date: 25/10/2022
Date deposited: 28/11/2022
ISSN (print): 0960-8524
ISSN (electronic): 1873-2976
Publisher: Elsevier Ltd
URL: https://doi.org/10.1016/j.biortech.2022.128216
DOI: 10.1016/j.biortech.2022.128216
PubMed id: 36347482
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