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Lookup NU author(s): Dr Emanuela Torelli
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
© 2023, The Author(s).Abstract: Foods contaminated by pathogens are responsible for foodborne diseases which have socioeconomic impacts. Many approaches have been extensively investigated to obtain specific and sensitive methods to detect pathogens in food, but they are often not easy to perform and require trained personnel. This work aims to propose a textile organic electrochemical transistor-based (OECT) biosensor to detect L. monocytogenes in food samples. The analyses were performed with culture-based methods, Listeria Precis™ method, PCR, and our textile OECT biosensor which used poly(3,4-ethylenedioxythiophene) (PEDOT):polystyrene sulfonate (PSS) (PEDOT:PSS) for doping the organic channel. Atomic force microscopy (AFM) was used to obtain topographic maps of the gold gate. The electrochemical activity on gate electrodes was measured and related to the concentration of DNA extracted from samples and hybridized to the specific capture probe immobilized onto the gold surface of the gate. This assay reached a limit of detection of 1.05 ng/μL, corresponding to 0.56 pM of L. monocytogenes ATCC 7644, and allowed the specific and rapid detection of L. monocytogenes in the analyzed samples. Keypoints: • Textile organic electrochemical transistors functionalized with a specific DNA probe • AFM topographic and surface potential maps of a functionalized gold gate surface • Comparison between the Listeria monocytogenes Precis™ method and an OECT biosensor
Author(s): Vizzini P, Beltrame E, Coppede N, Vurro F, Andreatta F, Torelli E, Manzano M
Publication type: Article
Publication status: Published
Journal: Applied Microbiology and Biotechnology
Year: 2023
Volume: 107
Issue: 11
Pages: 3789-3800
Print publication date: 01/06/2023
Online publication date: 05/05/2023
Acceptance date: 16/04/2023
Date deposited: 22/05/2023
ISSN (print): 0175-7598
ISSN (electronic): 1432-0614
Publisher: Springer Science and Business Media Deutschland GmbH
URL: https://doi.org/10.1007/s00253-023-12543-y
DOI: 10.1007/s00253-023-12543-y
PubMed id: 37145160
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