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Lookup NU author(s): Dr Krishan Mistry, Grant Richardson, Rob Smith, Professor Penny Lovat
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
© 2024 The Authors. Wound Repair and Regeneration published by Wiley Periodicals LLC on behalf of The Wound Healing Society.Chronic non-healing cutaneous wounds represent a major burden to patients and healthcare providers worldwide, emphasising the continued unmet need for credible and efficacious therapeutic approaches for wound healing. We have recently shown the potential for collagen peptides to promote proliferation and migration during cutaneous wound healing. In the present study, we demonstrate that the application of porcine-derived collagen peptides significantly increases keratinocyte and dermal fibroblast expression of integrin α2β1 and activation of an extracellular signal-related kinase (ERK)-focal adhesion kinase (FAK) signalling cascade during wound closure in vitro. SiRNA-mediated knockdown of integrin β1 impaired porcine-derived collagen peptide-induced wound closure and activation of ERK-FAK signalling in keratinocytes but did not impair ERK or FAK signalling in dermal fibroblasts, implying the activation of differing downstream signalling pathways. Studies in ex vivo human 3D skin equivalents subjected to punch biopsy-induced wounding confirmed the ability of porcine-derived collagen peptides to promote wound closure by enhancing re-epithelialisation. Collectively, these data highlight the translational and clinical potential for porcine-derived collagen peptides as a viable therapeutic approach to promote re-epithelialisation of superficial cutaneous wounds.
Author(s): Mistry K, Richardson G, Vleminckx S, Smith R, Gevaert E, Lovat PE
Publication type: Article
Publication status: Published
Journal: Wound Repair and Regeneration
Year: 2024
Pages: epub ahead of print
Online publication date: 04/04/2024
Acceptance date: 25/03/2024
Date deposited: 17/04/2024
ISSN (print): 1067-1927
ISSN (electronic): 1524-475X
Publisher: John Wiley and Sons Inc
URL: https://doi.org/10.1111/wrr.13177
DOI: 10.1111/wrr.13177
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