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Glutathione transferase pi its minimal promoter and downstream cis- acting element

Lookup NU author(s): Dr Ian Cowell

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Abstract

Fragments of the human glutathione S-transferase pi gene and 15 kb of its 5' flanking region have been fused to the chloramphenicol acetyl transferase (CAT) reporter gene. Transfection into a number of human cell lines (Hela, HepG2, MCF7 and EJ) has demonstrated that the AP1 binding site, located between nucleotides -58 and -65 (Cowell et al. 1988. Biochem. J. 255, 79-83), is essential for basal level promoter activity. We have also identified a positive cis-acting DNA element between nucleotides +8 and +72 which seems to be part of the promoter. No other regulatory activity was identified within the 17 kb analyzed


Publication metadata

Author(s): Xia CL, Cowell IG, Dixon KH, Pemble SE, Ketterer B, Taylor JB

Publication type: Article

Publication status: Published

Journal: Biochemical and Biophysical Research Communications

Year: 1991

Volume: 176

Issue: 1

Pages: 233-240

Print publication date: 15/04/1991

ISSN (print): 0006-291X

ISSN (electronic): 1090-2104

Publisher: Academic Press

URL: http://dx.doi.org/10.1016/0006-291X(91)90914-S

DOI: 10.1016/0006-291X(91)90914-S

PubMed id: 1850260


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