Toggle Main Menu Toggle Search

Open Access padlockePrints

Tenascin-C and human tendon degeneration

Lookup NU author(s): Emeritus Professor Tim Cawston

Downloads

Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


Abstract

We investigated the distribution of tenascin in supraspinatus tendons to determine whether an alteration in tenascin expression was associated with human tendon degeneration. Tenascin was present in all of the tendons studied, although with two distinct patterns of expression. First, tenascin was associated with organized, fibrous regions of the tendon matrix that were typical of the normal tendon structure. This distribution is consistent with a role for tenascin in collagen fibril organization, perhaps maintaining the interface between fibrils and adjacent structures. Second, although tenascin was generally absent from poorly organized matrix in degenerate tendons, it was strongly associated with some rounded cells in disorganized fibrocartilaginous regions that were more abundant in pathological specimens. Tenascin was also found around infiltrating blood vessels, with more intense staining associated with a mononuclear cell infiltrate. Western blotting of tendon extracts showed differences in tenascin isoform expression, with only the small (200-kd) tenascin isoform found in normal tendons. Degenerate tendons also expressed the 300-kd isoform, consistent with a role for the larger tenascin isoform in tendon disease, potentially stimulating tenocyte proliferation, cell rounding, and fibrocartilaginous change. Proteolytic fragments of tenascin were detected but only in ruptured tendons, an indication of matrix remodeling in degenerate tendons, with fragment sizes consistent with the activity of matrix metalloproteinase enzymes.


Publication metadata

Author(s): Riley GP, Harrall RL, Cawston TE, Hazleman BL, Mackie EJ

Publication type: Article

Publication status: Published

Journal: The American Journal of Pathology

Year: 1996

Volume: 149

Issue: 3

Pages: 933-943

Print publication date: 01/09/1996

ISSN (print): 0002-9440

ISSN (electronic): 1525-2191

Publisher: American Society for Investigative Pathology

URL: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1865170/


Share