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Inhibitors of Actin Filament Polymerisation Attenuate Force but not Global [Ca2+]i in Isolated Pressurised Resistance Arteries

Lookup NU author(s): Professor Caroline Austin, Professor Michael Taggart

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Abstract

Receptor-coupled contractile activation of arterial smooth muscle involves increases in intracellular calcium ([Ca2+]i) and subsequent alteration of myosin light chain phosphorylation. An additional mechanism whereby agonists could regulate vascular contractility may be alteration of actin filament dynamics. Therefore, in this study, we have investigated the influence of two inhibitors of actin filament polymerisation, cytochalasin D and latrunculin B, on the [Ca2+]i and force responsiveness of pressurised rat mesenteric arteries to α-adrenergic stimulation. Following cytochalasin D or latrunculin B treatment, phenylephrine-induced constrictions were significantly reduced to 11 ± 3.2% (n = 6) and 10 ± 4.4% (n = 6) of control, respectively, whereas [Ca2+]i remained at 98 ± 21% and 104 ± 7.0% of control, respectively. Such effects of cytochalasin D were not restricted to mesenteric small arteries. Cytochalasin D also significantly reduced the force, but not [Ca2+]i responses to agonist stimulation in other vascular (portal vein) and non-vascular (uterine) tissues. These data indicate that inhibitors of net actin polymerisation attenuate maximum agonist-induced force responsiveness without similar reductions in [Ca2+]i in pressurised resistance vessels and other smooth muscle tissues. This suggests that modulation of the dynamic equilibrium between filamentous F-actin and monomeric globular actin (G-actin) may be an important mechanism, acting independently of global [Ca2+]i homeostasis, to regulate the smooth muscle contractile state.


Publication metadata

Author(s): Shaw L, Ahmed S, Austin C, Taggart MJ

Publication type: Article

Publication status: Published

Journal: Journal of Vascular Research

Year: 2003

Volume: 40

Issue: 1

Pages: 1-10

Print publication date: 01/01/2003

ISSN (print): 1018-1172

ISSN (electronic): 1423-0135

Publisher: S. Karger AG

URL: http://dx.doi.org/10.1159/000068940

DOI: 10.1159/000068940


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