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Apoptosis in Porphyromonas gingivalis-specific T-cell lines

Lookup NU author(s): Dr John Taylor

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Abstract

Fluorescence-activated cell sorter analysis and transmission electron microscopy were used to determine the presence of apoptotic cells in Porphyromonas gingivalis-specific T-cell lines established from the peripheral blood of 10 P. gingivalis-infected individuals. P. gingivalis outer membrane antigens were presented to the T cells by autologous Epstein-Barr virus-transformed B cells for 6, 24, 48 and 72 h. Transmission electron microscopy demonstrated the presence of typical apoptotic cells in all cultures. Annexin V-positive cells were present at low concentrations at all 4 four periods. A mean of approximately 2-3% of the CD4 cells and 1-3.5% of the CD8 cells were annexin V-positive, with an increase to around 5.5% positive CD4 cells at 6 h in wells containing P. gingivalis compared with cultures not containing antigen. This difference was not, however, significant at the 0.05 level (P=0.073). The mean (±standard error) CD4:CD8 ratios of the T-cell lines when first established using peripheral blood mononuclear cells as antigen-presenting cells was significantly higher (5.2±1.1) than when transformed B cells were used as antigen-presenting cell (1.2±0.5). While this study has shown apoptosis occurring in the T-cell lines, it has not shown definitively that the reversion in the CD4:CD8 ratio in the P. gingivalis-specific T cells following antigen presentation by autologous Epstein-Barr virus-transformed B cells is due to apoptosis of a CD4 population. Alternatively, the reversion in the CD4:CD8 ratio could be due to a selective proliferation of the CD8 population which, in turn, could be relevant to the immunopathology of periodontal disease induced by P. gingivalis.


Publication metadata

Author(s): Taylor JJ; Gemmell E; Prajaneh S; Grieco DA; Seymour GJ

Publication type: Article

Publication status: Published

Journal: Oral Microbiology and Immunology

Year: 1999

Volume: 14

Issue: 6

Pages: 331-338

Print publication date: 01/12/1999

ISSN (print): 0902-0055

ISSN (electronic): 1399-302X

Publisher: John Wiley & Sons

URL: http://dx.doi.org/10.1034/j.1399-302X.1999.140601.x

DOI: 10.1034/j.1399-302X.1999.140601.x

PubMed id: 10895687


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