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Sequence analysis of 16S rRNA genes amplified from two ribosomal RNA gene clusters of Bifidobacterium bifidum

Lookup NU author(s): Junghyun Yoon, Professor Michael Goodfellow

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Abstract

Two rRNA gene clusters were detected in the genome of Bifidobacterium bifidum KCTC 3202(T) using Southern blot analysis. To analyse the sequences of the 16S rRNA genes from rrnA and rrnB, 16S rDNAs were amplified by PCR using DNA fragments purified from gel slices containing each of the rRNA gene clusters. The amplified 16S rDNAs from rrnA and rrnB were cloned into vectors and three clones of each gene sequenced. The resultant sequences were confirmed by direct sequencing of the 16S rDNAs from rrnA and rrnB. Sequence differences were not found between rrnA and rrnB in 1488 bp of the 16S rRNA genes.

Publication metadata

Author(s): Yoon J-H, Yim DK, Goodfellow M, Park Y-H

Publication type: Article

Publication status: Published

Journal: Antonie van Leeuwenhoek

Year: 1999

Volume: 75

Issue: 4

Pages: 329-333

ISSN (print): 0003-6072

ISSN (electronic): 1572-9699

Publisher: Springer Netherlands

URL: http://dx.doi.org/10.1023/A:1002025100444

DOI: 10.1023/A:1002025100444

PubMed id: 10510720

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