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Molecular characterization of a zebrafish TCF ETS-domain transcription factor

Lookup NU author(s): Dr Alan Hair, Alexios Galanis

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Abstract

The ternary complex factor (TCF) subfamily of ETS-transcription factors represent key nuclear targets of the MAP kinase pathways. Members of this subfamily are classified by the presence of several conserved domains for DNA binding, interaction with SRF, interaction with MAP kinases and transcriptional activation. In this study we have isolated a further member of this subfamily (TCF-1) from zebrafish. The protein product of zebrafish TCF-1 (zTCF-1), shares sequence similarity with the mammalian TCFs in all four conserved domains, with highest overall similarity to SAP-1. Zebrafish TCF-1 is expressed throughout zebrafish embryonic development and exhibits typical TCF DNA binding characteristics, with the B-box being required for interaction with SRF. Of the mammalian TCFs, its DNA binding specificity resembles Elk-1. zTCF-1 is a target for both the growth factor/mitogen-activated and stress-activated MAP kinase cascades in vitro and in vivo. However, differential targeting occurs, with the profile of its activation closely resembling that of mammalian SAP-1. Together, our results demonstrate that the TCFs have been functionally conserved during vertebrate development.


Publication metadata

Author(s): Brown LA, Yang S-H, Hair A, Galanis A, Sharrocks AD

Publication type: Article

Publication status: Published

Journal: Oncogene

Year: 1999

Volume: 18

Issue: 56

Pages: 7985-7993

Print publication date: 23/12/1999

ISSN (print): 0950-9232

ISSN (electronic): 1476-5594

Publisher: Nature Publishing Group

URL: http://dx.doi.org/10.1038/sj.onc.1203197

DOI: 10.1038/sj.onc.1203197

PubMed id: 10637509


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