Lookup NU author(s): Dr Trudy Netherwood,
Professor Anthony O'Donnell,
Professor Harry Gilbert
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The maximum in vivo transfer rate of plasmid pAMβ1 in the gut was 0.03 transconjugant per recipient cell, and this rate could be simulated in vitro only by forced filter mating. Transfer was not detected in liquid culture matings. Our findings demonstrate that in vitro methods, such as forced filter mating and liquid mating, underestimate the in vivo rates of gene transfer.
Author(s): Netherwood T, Bowden R, Harrison P, O'Donnell AG, Parker DS, Gilbert HJ
Publication type: Article
Publication status: Published
Journal: Applied and Environmental Microbiology
Print publication date: 01/11/1999
ISSN (print): 0099-2240
ISSN (electronic): 1098-5336
Publisher: American Society for Microbiology
PubMed id: 10543833