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Gene transfer in the gastrointestinal tract

Lookup NU author(s): Dr Trudy Netherwood, Professor Anthony O'Donnell, Professor Harry Gilbert

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Abstract

The maximum in vivo transfer rate of plasmid pAMĪ²1 in the gut was 0.03 transconjugant per recipient cell, and this rate could be simulated in vitro only by forced filter mating. Transfer was not detected in liquid culture matings. Our findings demonstrate that in vitro methods, such as forced filter mating and liquid mating, underestimate the in vivo rates of gene transfer.


Publication metadata

Author(s): Netherwood T, Bowden R, Harrison P, O'Donnell AG, Parker DS, Gilbert HJ

Publication type: Article

Publication status: Published

Journal: Applied and Environmental Microbiology

Year: 1999

Volume: 65

Issue: 11

Pages: 5139-5141

Print publication date: 01/11/1999

ISSN (print): 0099-2240

ISSN (electronic): 1098-5336

Publisher: American Society for Microbiology

URL: http://aem.asm.org/content/65/11/5139.abstract

PubMed id: 10543833


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