Toggle Main Menu Toggle Search

Open Access padlockePrints

Predictability of dorso-ventral asymmetry in the cleavage stage zebrafish embryo: an analysis using lithium sensitivity as a dorso-ventral marker

Lookup NU author(s): Emeritus Professor Michael Whitaker

Downloads

Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


Abstract

The dorso-ventral axis in zebrafish first becomes apparent at gastrulation, when the future ventral side appears thinner than the dorsal side. The exact time of establishment of the dorso-ventral axis is not known. We show here that the dorso-ventral axis is specified as early as the 32 cell stage. Using lithium as a marker for dorso-ventral asymmetry, we show that lithium-sensitivity is a characteristic of future ventral cell, but not future dorsal cells, and that there is an asymmetric lithium-sensitivity along the long axis of the 32 cell stage embryo. Consequently, the dorso-ventral axis corresponds to the long axis of the embryo. Because the effect of lithium treatment is short-lived, the dorso-ventral axis must be specified in zebrafish already at the 32 cell stage. Copyright (C) 1999 Elsevier Science Ireland Ltd.


Publication metadata

Author(s): Aanstad P, Whitaker M

Publication type: Article

Publication status: Published

Journal: Mechanisms of Development

Year: 1999

Volume: 88

Issue: 1

Pages: 33-41

Print publication date: 01/10/1999

ISSN (print): 0925-4773

ISSN (electronic): 1872-6356

Publisher: Elsevier

URL: http://dx.doi.org/10.1016/S0925-4773(99)00171-9

DOI: 10.1016/S0925-4773(99)00171-9

PubMed id: 10525186


Altmetrics

Altmetrics provided by Altmetric


Share